ABSTRACT. The present study aimed to evaluate the in vitro antimicrobial activity of pharmacologically important Adhatoda vasica plant extracts. Plant Adhatoda vasica L. Nees (Acanthaceae) commonly known as Malabar nut is an evergreen two – three m tall shrub, sometime used as. Adhatoda vasica (L.) (Acanthaceae) is used in the indigenous system of medicine in India. The alkaloid Vasicine was isolated from ethanolic extract of the .
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Classical Uses of Medicinal Plants. Author information Article notes Copyright and License information Disclaimer. Ignacimuthu123 C. After various durations of cultivation, the antimicrpbial in the medium was removed. Extraction Shade dried and coarsely powdered leaves of A. The physical and spectroscopic data were compared with those reported in the literature [ 2122 ]. Vasicine acetate exhibited good zone of inhibition against bacteria: The free radical scavenging activity was calculated as follows:.
Separation was carried out at room temperature. Structure of the compounds: Shade dried and coarsely powdered leaves of A. Investigation on antifeedant and anthelmentic potential of Adhatoda vasica nees.
Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper
The radical scavenging activity of Vasicine acetate at different concentrations is shown in Table 3. Ray of hope for cancer patients. DPPH 2,2-diphenylpicrylhydrazyl radical scavenging activity of Vasicine acetate was determined based on the method of Wang et al.
The plant is used in the indigenous system of medicine in India and is a well-known expectorant in both Ayurvedic and Unani Systems of Medicine [ 45 ]. Asian Pacific Journal of Tropical Biomedicine. The extract was filtered through Whatman No. The plant was identified by Dr. Accumulation of vasicine and vasicinone in tissue cultures of Adhatoda vasica and evaluation of the free radical-scavenging activities of the various crude extracts.
International Journal of Green Pharmacy. Vasicine acetate was obtained by acetylation of Vasicine recovered from A.
Cytotoxic A and antimicrobial effects of Methylobacterium sp. Kulkarni [ 24 ] reported that A. Cytotoxic effect of Vasicine acetate against A lung adenocarcinoma cancer cell line. The alkaloid Vasicine was isolated from ethanolic extract of the leaves of A.
Antibacterial activity of adhatoda vasica leaf extract
The emergence of drug-resistant pathogens and the increase in diseases affecting the immune system have greatly intensified the need to investigate new bioactive metabolites for potential pharmaceutical and industrial applications [ 12 ].
The solutions were filtered through a membrane filter pore size 0. Acanthaceae is used in the indigenous system of medicine in India. Al-Dhabi1 C. Two mobile phases A and B were used at flow rate of 1. Evaluation of antioxidant activity of isoferulic acid in vitro. HPLC chromatograms of the ethanol extract of the leaves of Adhatoda vasica.
The chemicals are given in delta scale with TMS as an internal standard. Cytotoxicity of each sample was expressed as IC 50 value. Conclusion Vasicine acetate was obtained by acetylation of Vasicine recovered from A.
Nature acts as a prominent reservoir for new activty novel therapeutics. Zones of inhibition were recorded in millimetres and the experiment was repeated twice. The estimation of Vasicine acetate content in the extract was performed using linear regression analysis. Antibacterial and antifungal activities were carried out using disc diffusion method [ 16 ].
Materials and Methods 2. Table 4 Cytotoxic effect of Vasicine acetate against A lung adenocarcinoma cancer cell line.